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. 2022 Nov 17;10(2):2204190. doi: 10.1002/advs.202204190

Figure 2.

Figure 2

Constitutive PPARγ acetylation in adipocytes impairs adipose plasticity. a) Schematic outline of aKQ mouse breeding strategy. b) Gene expression validation by qPCR in eWAT and BAT from WT and aKQ mice (n = 6, 6). Ribosomal Protein L23 (Rpl23) was used as the reference gene. c) Body weight of male WT and aKQ mice on chow diet feeding at ≈10 weeks old (n = 11, 11). d–f) Indirect calorimetric analyses of 14‐weeks‐old male WT and aKQ mice fed a chow diet at room temperature (n = 9, 6); d) Oxygen consumption (normalized to body weight); e) Heat production (normalized to body weight); f) RER. *p < 0.05 at multiple detection points. g–j) Male WT and aKQ mice subjected to calorie restriction (CR) (n = 6, 6); g) body weight changes during the first 3 weeks CR; h) body composition at 2 weeks of CR; i) sacrificed tissue mass at 4 weeks of CR; j) ITT after 3 weeks of CR. k–m) 4‐weeks‐old male WT and aKQ mice on HFD for 12 weeks (n = 5, 8); k) body weight curve; l) fat mass curve on HFD feeding; m) tissue weight at sacrifice. n) Schematic diagram of impaired adipose plasticity in aKQ mice. The schemes in (a) and (n) were created using BioRender. Data are presented as mean ± SEM, *p < 0.05, **p < 0.01 by two‐tailed Student's t‐test.