FIGURE 4.

Validation of long‐chain acyl‐coenzyme A synthases 5 (ACSL5)‐dependent roles after down‐regulation of ACSL5. Transcriptomic profiles of ACSL5 ^NC and ACSL5 ^KD with vehicle or lysophosphatidylcholine (lysoPC) were measured by bulk RNA‐seq, of which the heatmap of top 50 lipid‐associated differentially expressed genes (A), the exact levels of ACSL family member genes (B) and the signal pathway enrichment of those genes (C) were presented (n = 3 per group and repeated thrice). ACSL5‐dominated lipid metabolism gene networks using Cytoscape (D), heatmap of genes involved in fatty acid biosynthesis, degradation and elongation (E) and volcano plot of lipid‐related genes (F) between ACSL5 ^NC and ACSL5 ^KD cells treated with vehicle or lysoPC were calculated, on basis of up‐regulated genes with fold changes > 1.5 and down‐regulated genes with fold changes < 0.67 (p < .05). * and ** or # and ## stand for the p‐values less than .05 and .01, respectively, as compared with ACSL5 ^NC cells treated with vehicle or lysoPC. FASN: fatty acid synthase; OXSM: 3‐Oxoacyl‐ACP Synthase, mitochondrial; ACSL5: acyl‐CoA synthetase long‐chain family member 5; ACAT1: acetyl‐CoA acetyltransferase 1; ALDH9A1: aldehyde dehydrogenase 9 family member A1; ACADM: acyl‐CoA dehydrogenase medium chain; ECI2: enoyl‐CoA delta isomerase 2; HADHA: hydroxyacyl‐CoA dehydrogenase trifunctional multienzyme complex subunit alpha; HADHB: hydroxyacyl‐CoA dehydrogenase trifunctional multienzyme complex subunit beta; ELOVL4: ELOVL fatty acid elongase 4; PPT1: palmitoyl‐protein thioesterase 1; ACOT7: acyl‐CoA thioesterase 7; and ELOVL3: ELOVL fatty acid elongase 3