Fig. 1.

Inhibitory effects of water extracts of Mori Ramulus (MRWE) on hydrogen peroxide (H₂O₂)-mediated cytotoxicity and cell cycle arrest at the G2/M phase in L6 cells. Cells were treated with different concentrations of MRWE for 24 h (a) or treated with MRWE for 1 h, and then stimulated with H₂O₂ for 24 h (b–d). a and b The results of quantitative analysis of cell viability according to MTT assay were presented. c and d Cells were collected and analysis of cell cycle distribution was performed. c Representative flow histograms were presented. d The frequency of sub-G1 cells were presented. a, b and d The data were represented as mean ± standard deviation (SD) of three independent experiments. Significant differences compared to the control cells (*p < 0.05, **p < 0.01 and ***p < 0.001) or H₂O₂‑treated cells (^#p < 0.05, ^##p < 0.01 and ^###p < 0.001) were shown