Skip to main content
. 2022 Sep 23;39(1):115–125. doi: 10.1007/s43188-022-00149-x

Table 4.

The number of spermatogenic cells in seminiferous tubules of male mice treated with cyclophosphamide (CP) and/or China dust (CD)

Items Group
NC CP CD40 CP + CD20 CP + CD40
Stage II Spermatogonia 20.6 ± 2.88a 7.2 ± 1.92** 16.4 ± 3.44 4.4 ± 1.34**,† 3.8 ± 1.48**,†
Pachytene spermatocytes 51.0 ± 9.14 29.6 ± 8.11** 44.6 ± 5.86 18.8 ± 4.09**,† 16.0 ± 3.67**,††
Round spermatids 134.0 ± 21.90 125.8 ± 9.55 122.4 ± 12.30 112.8 ± 9.86 106.0 ± 15.79*
Elongated spermatids 180.0 ± 13.98 164.8 ± 22.53 165.8 ± 16.86 152.0 ± 31.99 157.0 ± 32.19
Sertoli cells 12.8 ± 1.92 11.8 ± 2.49 12.2 ± 2.28 12.2 ± 1.79 11.4 ± 2.07
Stage V Spermatogonia 34.2 ± 4.44 8.2 ± 4.21** 28.6 ± 4.51 6.0 ± 4.00** 5.8 ± 3.27**
Pachytene spermatocytes 60.0 ± 7.11 34.4 ± 9.13** 48.2 ± 3.77 21.2 ± 8.93** 17.2 ± 5.72**,†
Round spermatids 153.8 ± 20.78 106.6 ± 11.89** 123.4 ± 11.72 102.0 ± 9.92** 100.0 ± 7.07**
Elongated spermatids 205.8 ± 21.70 173.4 ± 23.75 202.6 ± 20.92 158.2 ± 35.39* 159.0 ± 40.08
Sertoli cells 13.6 ± 3.21 11.6 ± 1.14 13.0 ± 2.83 13.0 ± 1.58 11.2 ± 0.84
Stage VII Spermatogonia 2.0 ± 0.71 2.0 ± 0.71 2.0 ± 0.71 1.6 ± 0.89 2.2 ± 0.84
Preleptotene spermatocytes 45.8 ± 7.50 7.8 ± 1.92** 37.6 ± 2.97 3.4 ± 2.19**,† 2.6 ± 1.82**,††
Pachytene spermatocytes 55.0 ± 9.57 28.0 ± 14.63** 42.8 ± 5.85 29.0 ± 8.00 22.0 ± 7.35
Round spermatids 155.4 ± 27.75 123.8 ± 14.20 132.2 ± 19.24 120.8 ± 28.49* 112.8 ± 6.30*
Elongated spermatids 231.0 ± 36.80 202.4 ± 12.90 218.8 ± 18.38 189.0 ± 34.58 181.0 ± 35.94
Sertoli cells 13.6 ± 1.34 13.2 ± 2.78 12.2 ± 1.92 12.4 ± 1.95 12.2 ± 1.92
Stage XII Spermatogonia 2.2 ± 0.84 1.8 ± 0.84 2.0 ± 0.71 2.2 ± 0.84 1.8 ± 0.84
Zygotene spermatocytes 50.8 ± 4.55 11.6 ± 2.88** 46.8 ± 5.26 6.4 ± 1.52**,†† 4.4 ± 2.19**,††
Pachytene spermatocytes 65.2 ± 6.22 37.8 ± 13.05** 54.6 ± 5.18 43.8 ± 11.08** 32.0 ± 11.05**
Elongated spermatids 112.8 ± 13.37 101.0 ± 6.89 115.6 ± 6.80 103.4 ± 9.99 91.6 ± 7.13**
Sertoli cells 11.4 ± 1.52 12.2 ± 1.30 12.0 ± 2.00 11.8 ± 1.92 12.2 ± 2.77

a Values are presented as means ± SD. *p < 0.05 vs. NC group; **p < 0.01 vs. NC group; p < 0.05 vs. CP group; ††p < 0.01 vs. CP group, respectively