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Transmission IEM of S. pneumoniae CP1200. Polyclonal mouse antiserum to rPhpA-79 was used as the primary antibody. Eighteen-nanometer gold particle-labeled anti-mouse IgG and IgM were used as the secondary antibodies. Cells were photographed at a magnification of ×50,000. Panels: A, primary serum from a preimmune mouse; B, anti-rPhpA-79 primary serum.
