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Binding of tetra-, hexa-, and octa-valent mannose ligands to M1 macrophages
Biotinylated tetra-, hexa-, or octa-valent mannose ligands were complexed with AF488-labeled streptavidin and incubated with M1 macrophages. The MFI of AF488 was quantified using flow cytometry to determine the binding affinity of each ligand (n = 2; ∗p < 0.05; ∗∗∗p < 0.01; n.s., p > 0.05, two-way ANOVA analysis). The error bars represent standard deviation.
