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. 2022 Sep 15;31(1):269–281. doi: 10.1016/j.ymthe.2022.09.009

Figure 3.

Figure 3

Uptake of fluorescent-labeled mannose-siRNA conjugate by M1 macrophage

(A) Cy3-labeled tetra-mannose-siCD45 or tetra-GalNAc-siCD45 were incubated with M1 macrophages or HepG2 cells. The delivery of conjugates was determined by detection of Cy3 fluorescence under a fluorescence microscope. Cell nuclei were stained with DAPI. Scale bar, 100 μm.

(B) Expression of CD206 in M1 and HepG2 cells were measured by western blot. The blank lanes between the samples were removed to allow better comparison.

(C) M1 macrophages were treated with D-mannose or D-galactose before incubating with Cy3-tetra-mannose-siCD45. The delivery of conjugates was determined by detection of Cy3 fluorescence under a fluorescence microscope. Cell nuclei were stained with DAPI. Scale bar, 100 μm.