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. 2022 Sep 6;31(1):154–173. doi: 10.1016/j.ymthe.2022.08.023

Figure 2.

Figure 2

NLRC3 depletion in myeloid cells alleviated sepsis-induced immunosuppression

(A) Schematic overview of experimental design for (B) to (I). (B) Kaplan-Meier survival curves. All mice were monitored for 10 days after secondary P. aeruginosa challenge (n = 16 mice/group). (C–F) Bacterial loads in the lung and blood (C), ELISA for TNF-α, IL-1β, and IL-6 in lung homogenate (D), histopathological images of lung tissues (E), and histological injury scores (F); samples were collected at 24, 48, or 96 h after the secondary infection. Scale bar represents 100 μm (n = 6 mice/group). (G–I) Percentages of lung-infiltrating cells that were neutrophils (CD11b+ Gr-1+) or monocyte-macrophages (CD11b+ Gr-1−) (G and H). Expression levels of CD86, MHC II, and CD206 were detected on monocyte-macrophages (CD11b+ Gr-1−), with representative histogram with the corresponding median fluorescence intensity (MFI) (I). All samples were collected at 24 h after the infection challenge; assays were conducted by flow cytometry (n = 6 mice/group). Circles represent individual mice. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; NS, not significant (two-way ANOVA or Student’s t test and log-rank test for survival). See also Figures S4–S6.