Figure 7.

Advantage of FOXP3-overexpressing CD8 T cells in low-glucose conditions

(A and B) Eight-day in vitro-expanded Foxp3UP and mock (both GFP⁺) CD8 T cells were labeled with CTV dye and restimulated in vitro (72 h) with plate-bound anti-CD3 mAb in IL-2- and glucose-free DMEM supplemented with normal or low glucose concentration and FCS in the presence or absence of 2-DG, Eto, or Omy. (A) Graphs showing the percentage of proliferating cells in transduced (GPF⁺) cells and representative histograms of the CTV dilution assay. As reference, cells left without stimulation (Ctr) are shown (dotted histogram). (B) Proliferation inhibition index calculated as CTV MFI of inhibitor-treated cells/CTV MFI of inhibitor-free cells. (C) CTV-labeled cells cultured as in (A) and (B), but in hypoxia (1% O₂). Percentage of proliferating cells in transduced (GPF⁺) cells. Data are presented as mean ± SD. One experiment was representative of two experiments (A–C).