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. Author manuscript; available in PMC: 2023 Jan 14.
Published in final edited form as: Biochemistry. 2022 Sep 29;61(20):2206–2220. doi: 10.1021/acs.biochem.2c00495

Figure 4.

Figure 4.

Vesicle dye leak analysis monitored by 6-carboxyfluorescein (6-FAM) dye on: (a) TBE LUVs incubated with 10 μM Aβ monomers (Δ) or 2 μM isolated Aβ fibrils generated from the same liposomes (■, green); 50% GM1-enriched TBE LUVs incubated with 10μM Aβ monomers (▼, green); or 2 μM isolated Aβ fibrils generated from 50% GM1-enriched liposomes (●, red); (b) TBE LUVs incubated with 2 μM sonicated Aβ fibrils generated in the presence TBE liposomes (■, green) or 50% GM1-enriched TBE LUVs incubated with 2 μM sonicated Aβ fibrils generated in the presence of 50% GM1-enriched liposomes (●, red); (c) TBE LUVs incubated with 2 μM isolated Aβ fibrils generated in the absence of liposomes (■, blue) or 50% GM1-enriched LUVs incubated with 2 μM isolated Aβ fibrils generated in the absence of liposomes (●, brown); (d) samples in (c) but sonicated; (e) ThT fluorescence of 10μM Aβ monomers in the presence of 50% GM1-enriched TBE LUVs (◀, blue) and 50% GM3-enriched TBE LUVs (■, black); 6-FAM dye leakage of 50% GM1-enriched TBE LUVs (▼, green) and 50% GM3-enriched TBE LUVs (○, green) in the presence of 10 μM Aβ monomers; (f) zoomed-in image of Figure 4e showing the initial 6 h of the reaction.