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. Author manuscript; available in PMC: 2023 Jan 14.

Published in final edited form as: Biochemistry. 2022 Sep 29;61(20):2206–2220. doi: 10.1021/acs.biochem.2c00495

Figure 5. — Computational fits of 6-carboxyfluorescein dye leak assay of TBE LUVs (a) with 50% GM1 and 10 μM Aβ monomers (○), without GM1 and 10 μM Aβ monomers (■, black), with 50% GM1 and 2 μM Aβ fibrils (▶, purple), without GM1 and 2 μM Aβ fibril (◆, green) (b) with 50% GM1 and 10 μM Aβ monomers (○), without GM1 and 10 μM Aβ monomers (■), with 50% GM1 and 2 μM sonicated Aβ fibrils (▼, purple), without GM1 and 2 μM sonicated Aβ fibril (, green). (c) Normalized ThT fluorescence kinetics of buffered 10 μM Aβ without (■; control) or with TBE LUVs each of them enriched with 50 (▲, blue) % GM1 ganglioside (by wt.) or without (◆, brown) GM1 in the presence of 50 mM NaCl in 10 mM sodium phosphate buffer pH 8.00; Aβ monomers (A1 (black), oligomers A2 (red) and A3 (blue), and fibrils F (pink)) distribution plots for first 5 h from the start of reactions of Aβ monomers with TBE LUVs, (d) no GM1, (e) 50% GM1 or (f) without LUVs.

Inline graphic — Computational fits of 6-carboxyfluorescein dye leak assay of TBE LUVs (a) with 50% GM1 and 10 μM Aβ monomers (○), without GM1 and 10 μM Aβ monomers (■, black), with 50% GM1 and 2 μM Aβ fibrils (▶, purple), without GM1 and 2 μM Aβ fibril (◆, green) (b) with 50% GM1 and 10 μM Aβ monomers (○), without GM1 and 10 μM Aβ monomers (■), with 50% GM1 and 2 μM sonicated Aβ fibrils (▼, purple), without GM1 and 2 μM sonicated Aβ fibril (, green). (c) Normalized ThT fluorescence kinetics of buffered 10 μM Aβ without (■; control) or with TBE LUVs each of them enriched with 50 (▲, blue) % GM1 ganglioside (by wt.) or without (◆, brown) GM1 in the presence of 50 mM NaCl in 10 mM sodium phosphate buffer pH 8.00; Aβ monomers (A1 (black), oligomers A2 (red) and A3 (blue), and fibrils F (pink)) distribution plots for first 5 h from the start of reactions of Aβ monomers with TBE LUVs, (d) no GM1, (e) 50% GM1 or (f) without LUVs.