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Western blot of whole-cell lysates of strain A950006 and clones. The lysates were probed with E. coli-absorbed rabbit serum 25D. Lanes: 1, E. coli DH5α containing pGJB103; 2, E. coli DH5α clone 6 containing hmw; 3, H. influenzae Rdrec1 containing pGJB103; 4, H. influenzae Rdrec1 clone 6 containing hmw; 5, strain A950006; 6, A950006 (hmw::kan); 7, strain 12-2 (HMW1 positive and HMW2 negative) 8, strain 12-10 (HMW1 negative and HMW2 positive).
