TABLE 3.
Grouping of 32 adherent H. influenzae isolates based on adherence properties
| Adherence toa:
|
No. of isolates | Adherence pattern | No. of isolates
|
|||||
|---|---|---|---|---|---|---|---|---|
| Chang cells
|
NCI-H292 cells
|
hmw | HMW | HMW MAbs | ||||
| Without dextran sulfate | With dextran sulfate | Without dextran sulfate | With dextran sulfate | |||||
| ++ | − | +++ | − | 16 | Ib (HMW1)c | 13d | 13d | 9 |
| − | − | +++ | +++ | 4 | II (HMW2)c | 4 | 4 | 0 |
| ++ | ++ | − | − | 4 | III | 0 | 0 | 0 |
| ++ | ++ | +++ | − | 4 | IV | 4 | 4 | 4 |
| ++ | ++ | ++ | ++ | 4 | V | 1 | 1 | 1 |
| Total | 32 | 22 | 22 | 15 | ||||
a
−, <5 bacteria per cell; ++, 10 to 50 bacteria per cell; +++, >50 bacteria per cell.
b
Two isolates did not adhere to Chang epithelial cells.
c
This adherence pattern correlated with HMW1- or HMW2-mediated adherence of strain 12, as indicated.
d
One isolate was hmw positive and HMW negative, another was hmw negative and HMW positive.