FIG. 2.

Ab reactivity on sporozoite and liver stages. (A) Western blot analysis of 17XNL sporozoites extracts with human Abs immunopurified on the recombinant protein GST-PC (LSA3-Cterm) (lane 1) or human Abs immunopurified on the recombinant protein β-Gal-DG671 (SALSA antigen) used as control (lane 2). Masses are shown in kilodaltons. ∗, reactivity with a 205-kDa polypeptide. IFAT was performed with human anti-β-Gal-DG729 (LSA3-Nterm) immunopurified Abs on P. yoelii sporozoites isolated from salivary glands of infected Anopheles stephensi mosquitoes (B) and on a cryosection of P. yoelii liver schizont (44-h development) obtained in C3H/Hej mice (C). (D) A direct immunofluorescence assay was performed on the suspension injected into mice (Table 3), showing the detachment of the sporozoite membrane induced by anti-LSA3 antibodies upon live P. yoelii sporozoites. White bars, 10 μm.