| Antibodies |
| Rabbit anti-GFP |
Thermo Fisher |
Cat #A11122; RRID: AB_221569 |
| Rabbit anti-HA |
Thermo Fisher |
Cat #MA5-27915; RRID: AB_2744968 |
| Rabbit anti-FLAG |
Thermo Fisher |
Cat #740001; RRID: AB_2610628 |
| Mouse anti-HA |
Thermo Fisher |
Cat #32-6700; RRID: AB_2533092 |
| Rabbit anti-NeuN |
Abcam |
Cat #ab177487; RRID: AB_2532109 |
| Rabbit anti-S100b |
Abcam |
Cat #ab52642; RRID: AB_882426 |
| Mouse anti-rhodopsin |
Thermo Fisher |
Cat #MA1-722; RRID: AB_325050 |
| Goat anti-mouse Alexa Fluor Plus 488 |
Thermo Fisher |
Cat #A32723; RRID: AB_2633275 |
| Goat anti-rabbit Alexa Fluor Plus 594 |
Thermo Fisher |
Cat #A32740; RRID: AB_2762824 |
| Goat anti-rabbit Alexa Fluor Plus 488 |
Thermo Fisher |
Cat #A32731; RRID: AB_2633280 |
| Donkey anti-mouse Alexa Fluor Plus 594 |
Thermo Fisher |
Cat #A32744; RRID: AB_2762826 |
| Bacterial and Virus Strains |
| Endura chemically competent cells |
Lucigen |
Cat #60240-2 |
| Chemicals, Peptides, and Recombinant Proteins |
| RNAlater stabilization solution |
Thermo Fisher |
Cat #AM7024 |
| DMEM, high glucose, GlutaMAX Supplement, HEPES |
Thermo Fisher |
Cat #10564029 |
| Fetal bovine serum, certified, One Shot format |
Thermo Fisher |
Cat #A3160402 |
| PEI MAX |
Polysciences |
Cat #24765-1 |
| TRIzol reagent |
Thermo Fisher |
Cat #15596026 |
| QuickExtract DNA extract solution |
Lucigen |
Cat #QE09050 |
| Tissue-Tek O.C.T. compound |
Sakura Finetek |
Cat #4583 |
| Superfrost Plus slides |
VWR |
Cat #48311-703 |
| Normal goat serum |
Jackson ImmunoResearch |
Cat #005-000-121 |
| Hoechst 33342 |
Thermo Fisher |
Cat #H3570 |
| VECTASHIELD antifade mounting medium |
Vector Laboratories |
Cat #H-1400-10 |
| VectaMount permanent mounting medium |
Vector Laboratories |
Cat #H-5000-60 |
| CitriSolv |
Decon Labs |
Cat #1601 |
| Critical Commercial Assays |
| SuperScript IV reverse transcriptase |
Thermo Fisher |
Cat #18090050 |
| NEBuilder HiFi DNA assembly master mix |
New England Biolabs |
Cat # E2621 |
| Mouse GAPDH mRNA taqman assay |
Integrated DNA Technologies |
Cat #Mm.PT.39a.1 |
| Oligo d(T)25 magnetic beads |
New England Biolabs |
Cat #S1419S |
| TURBO DNase |
Thermo Fisher |
Cat #AM2239 |
| Q5 High-Fidelity 2X Master Mix |
New England Biolabs |
Cat #M0492 |
| HRP/DAB micropolymer IHC detection kit |
Abcam |
Cat #ab236466 |
| Mouse on Mouse (M.O.M.) immunodetection kit |
Vector Laboratories |
Cat #BMK-2202 |
| H&E staining kit |
Abcam |
Cat #ab245880 |
| TrueVIEW autofluorescence quenching kit |
Vector Laboratories |
Cat #SP-8400-15 |
| Deposited Data |
| NGS data |
NCBI sequence read archive |
Bioproject ID #PRJNA895493 |
| Experimental Models: Cell Lines |
| HEK293 |
ATCC |
Cat #CRL-1573 |
| Experimental Models: Organisms/Strains |
| Mouse: C57BL/6J |
Jackson Laboratory |
RRID: IMSR_JAX:000664 |
| Mouse: BALB/cJ |
Jackson Laboratory |
RRID: IMSR_JAX:000651 |
| Cynomolgus macaque |
Biomere Biomedical Research Models |
N/A |
| Oligonucleotides |
| Taqman assay primers and probes |
See Table S4 for a list of primer and probe sequences |
N/A |
| Recombinant DNA |
| Helper plasmid |
Aldevron |
Cat #pALD-X80 |
| Rep-AAP plasmid |
Dr. Benjamin Deverman |
N/A |
| Software and Algorithms |
| CellProfiler v4.2 |
Broad Institute |
doi.org/10.1186/s12859-021-04344-9
|
| GraphPad Prism v9 |
GraphPad Software |
https://www.graphpad.com/scientific-software/prism/
|
| NGS analysis code |
In house |
10.5281/zenodo.7262232
|
