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. Author manuscript; available in PMC: 2023 Jul 1.
Published in final edited form as: Nat Chem. 2022 Oct 10;15(1):33–42. doi: 10.1038/s41557-022-01059-z

Fig. 4. Genetic incorporation of SFY into proteins in E. coli.

Fig. 4.

a) Structure of SFY. b) Amino acid sequences of the evolved MmSFYRS, MaSFYRS, and the corresponding WT PylRS. c) Western blot analysis of SFY incorporation into sfGFP(2TAG) by Mm-tRNAPyl/MmSFYRS pair. d) ESI-TOF MS spectrum of intact sfGFP(2SFY) protein expressed by Mm-tRNAPyl/MmSFYRS pair. e) Tandem MS spectrum of Z(24SFY) expressed by Mm-tRNAPyl/MmSFYRS pair. U represents SFY. f) Western blot analysis of SFY incorporation into sfGFP(2TAG) by Ma-tRNAPyl/MaSFYRS pair. g) ESI-TOF MS spectrum of intact sfGFP(2SFY) protein expressed by Ma-tRNAPyl/MaSFYRS pair.