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. Author manuscript; available in PMC: 2023 Jul 1.
Published in final edited form as: Nat Chem. 2022 Oct 10;15(1):33–42. doi: 10.1038/s41557-022-01059-z

Fig. 6. Siglec-7v(SFY) enhanced NK cell killing of cancer cells.

Fig. 6.

a) Scheme showing the use of Siglec-7v(127SFY) to block the interaction between sialoglycan on tumor cell surface and Siglec-7 of NK cells. Decreasing the inhibitory signal of Siglec-7 on NK cells would enhance NK killing of tumor cells. b-d) Cytotoxicity assay of three hypersialylated cancer cell lines showed that Siglec-7v(127SFY) enhanced NK-92 cell killing over the WT Siglec-7v. The line and error bar represent mean ± SEM; n = 3 independent batches of Siglec-7v proteins. * p < 0.05; ** p < 0.01; *** p < 0.001; NS, not significant; two-sided t test. P values in panel b: 0.00086 for 3 μM, 0.014 for 6 μM, 0.00045 for 12 μM, and 0.018 for 30 μM. P values in panel c: 0.30 for 3 μM, 0.0019 for 6 μM, 0.0040 for 12 μM, and 0.000043 for 30 μM. P values in panel d: 0.44 for 3 μM, 0.049 for 6 μM, 0.0038 for 12 μM, and 0.014 for 30 μM.