Table 2.

Immunohistochemical (IHC) and Molecular Findings

Case	IHC (+)	IHC (−)	TRAF7 (NM_032271) mutation	TRAF7 mutation variant allele frequency	Copy number alterations	Sarcoma methylation classifier	Calibration score
1	SMA	Desmin, S100, melan-A, beta-catenin, MUC4, CD34, keratins, STAT6, beta catenin	c.1192C>T (p.P398S), c.1730A>G (p.Y577S)	6.1%, 8.0%	CDKN2A/B loss	Undifferentiated sarcoma (USARC)	0.79771
2	CD34, SMA (focal), GLUT1	SOX10, S100, EMA, pan-NTRK, ALK-1, MUC4, STAT6, desmin, beta-catenin and ER.	c.1606G>A (p.G536S)	21.5%	1p, 12q12–13, 16q arm level loss	Undifferentiated sarcoma (USARC)	0.98635
3	WT1, CD34, PR, scattered MDM2	CK, histiocytic CD1a CD163 CD68, calretinin, melanocytic markers, PAX8, SMA, desmin, ALK D5F3, SMARCA4 retained, SMARCB1 retained, CDK4	c.1896C>G (p.N632K)	14.2%	12q arm level gain, 20q13 gain, 16q12 gain	Undifferentiated sarcoma (USARC)	0.33081