Fig. 7.

Immunofluorescence staining for M1 polarization. Pan macrophage was stained with CD68 (red), M1 macrophage was stained with iNOS (green), and nucleus was stained with DAPI (blue). a) Representative images of CD68 and iNOS double staining of the wound tissues in the control, MgH₂ powder and MN-MgH₂ groups after 3, 7 and 14 days of treatment, respectively. b) Quantification of the percentage of CD68⁺iNOS⁺ cells in each group after 3, 7 and 14 days of treatment, respectively. n = 3, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.