Fig. 9.

In vivo investigation on the tissue regeneration, collagen fibrils repair, cell proliferation and angiogenesis efficacy of MN-MgH₂. a) H&E and Masson's trichrome staining of wound tissues in control, MgH₂ powder and MN-MgH₂ groups after 14 days of treatment. Green lines indicate fat cavitation. b) Representative images of anti-CD31 (red) immunofluorescence staining of dermis layer in wound area in control, MgH₂ powder and MN-MgH₂ groups after 14 days of treatment. Nucleus was stained with DAPI (blue). c) Representative images of anti-Ki67 (red) and immunofluorescence staining of dermis layer in wound area in control, MgH₂ powder and MN-MgH₂ groups after 14 days of treatment. Nucleus was stained with DAPI (blue). d) Quantification of the capillary density of dermis layer in wound area in each group. e) Quantification of the cell proliferation rate of wound tissues in the epidermis in each group. f) Quantification of cell proliferation rate of wound tissues in the dermis in each group. n = 3, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.