Sexually dimorphic response to high-fat die in Ts65Dn mice. A) Body weight of male Ts65Dn and euploid (Eu) mice over time fed a high-fat diet. B) Body composition analysis of absolute and relative (%) fat and lean mass in male Ts65Dn (n = 15) and euploid (n = 12) mice. C) Body weight of female Ts65Dn and euploid (Eu) mice over time fed a high-fat diet. D) Body composition analysis of absolute and relative (%) fat and lean mass in female Ts65Dn (n = 10) and euploid (n = 9) mice. E-G) Food intake (E), total physical activity level (F), and energy expenditure (G) of male Ts65Dn and euploid mice across the circadian cycle. Bar graphs indicate cumulative food intake, physical activity, and energy expenditure in the dark and light photocycle. H-J) Food intake (H), total physical activity level (I), and energy expenditure (J) of female Ts65Dn and euploid mice across the circadian cycle. Bar graphs indicate cumulative food intake, physical activity, and energy expenditure in the dark and light photocycle. All data are presented as mean ± SEM. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001. For body weight over time, data were analyzed by 2-way ANOVA with Sidek post hoc tests.