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. 2023 Jan 18;44(1):63–77. doi: 10.24272/j.issn.2095-8137.2022.167

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This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Lipid accumulation and elevated apoptosis in scd ^-/- livers

A: Scanning electron micrographs of WT and scd ^-/- liver sections showing increased lipid droplets in mutant livers. Scale bars: 10 μm. B: Scanning electron micrographs of WT and scd ^-/- liver sections showing shrunken mitochondria in mutant liver cells. Scale bars, 2 μm. C: Expression levels of scd, rxraa, elovl2, and elovl5 in scd ^-/- and scd ^-/- /Tg(CMV:scd) livers at 3 mpf (RT-qPCR). D: Expression levels of fatty acid synthesis-related genes dgat2, srebf1, acaca, fasn, and acc1 were significantly lower in scd ^-/- /Tg(CMV:scd) livers than in scd ^-/- livers at 3 mpf (RT-qPCR). E: Expression levels of oxidation-related genes pparαa, pparαb, acox3, and cpt1 in scd ^-/- and scd ^-/- /Tg(CMV:scd) livers at 3 mpf (RT-qPCR). F: Expression levels of transfer-related genes apoa1 and apob in scd ^-/- and scd ^-/- /Tg(CMV:scd) livers at 3 mpf (RT-qPCR). G: Expression levels of oxidative phosphorylation-related genes ndufab1, ndufa10, and ndufa9a in scd ^-/- and scd ^-/- /Tg(CMV:scd) livers at 3 mpf (RT-qPCR). H: Detection of liver cell apoptosis in WT, scd ^-/-, and scd ^-/- /Tg(CMV:scd) fish ( n=3, two sections for each sample) at 3 mpf with TUNEL staining, with DAPI blue indicating nucleus and TUNEL green indicating apoptosis signal. Scale bars: 100 μm. H1: Number of apoptotic cells (%) ( n=3, two sections for each sample). I: Immunofluorescence detection of WT and scd ^-/- liver sections ( n=3, two sections for each sample) with Caspase-3 antibody. Scale bars: 25 μm. I1: Number of Caspase-3-positive cells (%) ( n=3, two sections for each sample). All values are mean± SEM. Student t-tests were used. ^*: P<0.05; ^**: P<0.01; ^***: P<0.001; ns: No significance.