DMEM for cell culture

Reagent	Final concentration	Final amount
Dulbecco’s Modified Eagle’s Medium (high glucose)	N/A	839 mL
Glutamine	2 mM	1 mL
Penicillin-streptomycin	100×	10 mL
Equine Serum	10% [Vol/Vol]	100 mL
Fetal Bovine Serum	5% [Vol/Vol]	50 mL
Total	N/A	1000 mL