NuPAGE loading buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| NuPAGE LDS buffer | N/A | 250 μL |
| 1 M dithiothreitol | 50 mM | 50 μL |
| ddH2O | N/A | 700 μL |
| Total | N/A | 1 mL |
Prepare fresh.
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