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. 2022 Dec 16;102(3):102430. doi: 10.1016/j.psj.2022.102430

Figure 8.

Figure 8

Effect of CX extract on PI3K/AKT and RAS/ERK signaling pathway, HIF1α and VEGFA proteins levels in GCs. (A) Western blot to detect the expression of PI3K/AKT signaling pathway in GCs after CX extract treated, quantified according to the western blot. β-actin was included as a loading control. (B) Western blot to detect the expression of RAS/ERK signaling pathway in GCs after CX extract treated, quantified according to the western blot. β-actin was included as a loading control. (C) Western blot to detect the expression of HIF1α and VEGFA in GCs after CX extract treated, quantified according to the western blot. β-actin was included as a loading control. (D) Immunofluorescence staining of HIF1α in CX extract-treated GCs. Scale bar = 100μm. (E) Immunofluorescence staining of VEGFA in CX extract-treated GCs. Scale bar = 100μm. (F) Elisa to detect the concentration of VEGFA in GCs medium (pg/mL) after CX extract treated. (G) Photographs of capillary-like structures in different groups of FMECs after the addition of GCs mediums (CX extract treated) were shown. Image J software is used to measure the capillary network. Histograms, respectively, on behalf of the number of master junctions and total length. Scale bar = 100 μm. All experiments were performed in triplicate, and the data are the mean ± S.E.M (*P < 0.05, ⁎⁎P < 0.01, ⁎⁎⁎P < 0.001).