Reagent or Resource	Source	Identifier
Antibodies
Anti‐FASN (clone G‐11)	Santa Cruz	CAT# sc‐48357
Anti‐ACC1	ProteinTech	CAT# 21923‐1‐AP
Anti‐ACC2 (clone D5B9)	Cell Signaling	CAT# 8578
Anti‐KAR	Abcam	CAT# ab236990
Anti‐TECR	Sigma	CAT# HPA056488
Anti‐SPTLC1	ProteinTech	CAT#15376‐1‐AP
Anti‐β‐Actin	Abclonal	CAT# AC026
Anti‐β‐Tublin	Abclonal	CAT# AC008
Anti‐Bcl2	ProteinTech	CAT# 12789‐1‐AP
Anti‐Bcl‐xL	ProteinTech	CAT# 26967‐1‐AP
Goat Anti‐Mouse	Jackson	CAT# 115‐035‐003
Goat Anti‐Rabbit	Jackson	CAT# 111‐035‐003
Chemicals
FBS	Gemini	CAT# 900‐108
Delipidated FBS	PAN	CAT# P30‐3402
Dialyzed FBS	This paper	N/A
Dialyzed delipidated FBS	This paper	N/A
Matrigel	BD Biosciences	CAT# 356234
Penicillin and streptomycin	Gibco	CAT# 15140122
D‐GLUCOSE (U‐13C6)	CIL	CAT# CLM‐1396‐1
DMEM power	Caisson	CAT# DMP52‐10LT
Bovine Serum Albumin	Sigma	CAT# A8806
Lipofectamine™ 3000 Reagent	Invitrogen	CAT# L3000‐015
Acetyl‐Coenzyme A	Sigma	CAT# 10101893001
Coenzyme A hydrate	Sigma	CAT# C4282
Malonyl coenzyme A lithium salt	Sigma	CAT# M4263
Palmitate	Sigma	CAT# P0500
Stearate	Sigma	CAT# S4751
Arachidate	Sigma	CAT# A3631
Oleate	Sigma	CAT# O1008
Linoleate	Sigma	CAT# L1012
α‐Linolenate	Sigma	CAT# L2376
z‐VAD‐FMK	MCE	CAT# HY‐16658B
Necrostain‐1	Selleck	CAT# S8037
Disulfiram	MCE	CAT# HY‐B0240
Ferrostain‐1	MCE	CAT# S7243
Trolox	MCE	CAT# HY‐101445
N‐Acetyl‐l‐cysteine	Selleck	CAT# S1623
Deferoxamine mesylate	Selleck	CAT# S5742
Tween 80	MCE	CAT# HY‐Y1891
Methyl cellulose	MCE	CAT# HY‐125861
JC‐1	Abcam	CAT# ab141387
H2DCFDA	MCE	CAT# HY‐D0940
Mito Tracker	Invitrogen	CAT# M22425
Hoechst33258	Solarbio	Cat# C0021
Critical Commercial Assays
CellTiter‐Glo® Luminescent Cell Viability Assay	Promega	Cat# G7571
Experimental models: cell lines
Human: MDA‐MB‐231 (female, 51 years old)	ATCC	Cat# HTB‐26
Human: HeLa (female, 31 years old)	ATCC	Cat# CCL‐2
Human: HEK293T (female, fetus)	ATCC	Cat# CRL‐3216
Human: MCF7 (female, 69 years old)	ATCC	Cat# HTB‐22
Human: MCF‐10A (female, 36 years old)	ATCC	Cat# CRL‐10317
Experimental Models: Organisms/Strains
Mouse: Nude mice (female, 6–8 weeks old)	Experimental Animal Center of Nanjing Biomedical Research Institute	N/A
Recombinant DNA
pCDH‐TECR	This paper	N/A
pLentiGuide‐sgACC1‐1	This paper	N/A
pLentiGuide‐sgACC1‐2	This paper	N/A
pLentiGuide‐sgACC2‐1	This paper	N/A
pLentiGuide‐sgACC2‐2	This paper	N/A
pLentiGuide‐sgFASN‐1	This paper	N/A
pLentiGuide‐sgFASN‐2	This paper	N/A
pLentiGuide‐sgKAR‐1	This paper	N/A
pLentiGuide‐sgKAR‐2	This paper	N/A
pLentiGuide‐sgTECR‐1	This paper	N/A
pLentiGuide‐sgTECR‐2	This paper	N/A
lentiCRISPRv2‐sgTECR‐1	This paper	N/A
lentiCRISPRv2‐sgTECR‐2	This paper	N/A
lentiCRISPRv2‐sgSPTLC1‐1	This paper	N/A
lentiCRISPRv2‐sgSPTLC1‐2	This paper	N/A
Sequences of Oligonucleotides for sgRNA Constructs
ACC1‐sg1: GAAGACCTTAAAGCCAATGC	This paper	N/A
ACC1‐sg2: CAACAACAACTATGCAAATG	This paper	N/A
ACC2‐sg1: TACCTGCACGGGGATTCTCT	This paper	N/A
ACC2‐sg2: CGTTGTCGCCCAGACGCTAC	This paper	N/A
FASN‐sg1: CGACCCACCTCCGTCCACGA	This paper	N/A
FASN‐sg2: TACGCCACCATCCTGAACGC	This paper	N/A
KAR‐sg1: TGATGCAAAGTCAACAGCAA	This paper	N/A
KAR‐sg2: CCTAGAAGAAAAATTCAAAG	This paper	N/A
TECR‐sg1: AATATGACTTTACGTCCAGT	This paper	N/A
TECR‐sg2: TGTACTCACTGGGGTCCAGG	This paper	N/A
SPTLC1‐sg1: GTTGGATAACCCTAGGGTTA	This paper	N/A
SPTLC1‐sg2: ACGATGTTGTAGTTGAGAGC	This paper	N/A