Figure EV1. Commitment to mating pheromone occurs at Whi5 exit.

Cells were grown for 120 min on glucose minimal medium and then exposed to 1 μM α‐factor. x‐axis depicts the fraction of Whi5 that had been exported (relative to the peak value in the last G1) at the time point of the switch. Open circles represent 244 individual cells that either directly arrest in G1 (y = 1) or complete one more division before inducing a mating arrest (y = 0). A logistic regression was performed on these data to estimate the probability of arrest based on nuclear Whi5. Black line represents the result of the regression; the shaded areas represent the 95% confidence intervals.