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. 2022 Nov 23;42(2):e110321. doi: 10.15252/embj.2021110321

Figure EV3. The Swe1 kinase is not essential for Whi5 re‐entries.

Figure EV3

  1. We constructed a swe1 deletion mutant and monitored its Whi5 response to starvation (wild‐type control is the same data as in Fig 5A). Depicted is the fraction of cells that re‐imports Whi5 when starved within 15 min after Start. Numbers over the bars indicate total number of cells that were analyzed.
  2. Experimental setup for (C): Cells in liquid culture were arrested in G1 (time point 1) as described in Fig 6A. Cells were released by inducing Cln1 expression. After 45 min (2), half of the culture was transferred to starvation medium (3), and the other half continued to progress until 90 min (4). These cells were then also transferred to starvation medium (5).
  3. Cells from the experiment described in (B) were sampled at the indicated times and protein lysates were used for Western blotting. Upper blot shows the signal of a commercial antibody against phosphorylated tyrosine 19 on CDK. After the detection, the blot was stripped and re‐analyzed (lower blot) with an antibody detecting the V5‐tagged total CDK1 (Cdc28).