Fig. 4.

Effect of malonate infusion in a mouse stroke model of tMCAO followed by reperfusion. (A) Mice were exposed by tMCAO to 45 min of ischemia and either saline (0.9 or 5%) or disodium malonate (DSM) 320 or 640 mg/kg was given intravenously starting 10 min before reperfusion until 10 min after reperfusion. Two hours after reperfusion mice well culled and TTC staining was performed. Typical data are shown. (B) Quantification of infarcts size from experiments carried out as in (A). Number of mice per group are shown in the bars. Data are presented as mean ± SEM and *p < 0.05 by Kruskal Wallis and Dunn's test. (C, D) Mice were treated as described in (A) and 10 min after reperfusion the brain circulation was cleared of blood with saline by cardiac perfusion. Then contralateral and ipsilateral brain hemispheres (C) and CSF (D) were isolated and malonate content determined by LC-MS/MS. Number of mice per group are shown above or within the bars. Data are presented as mean ± SEM.