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. Author manuscript; available in PMC: 2024 Jan 3.
Published in final edited form as: Cell Metab. 2022 Dec 16;35(1):134–149.e6. doi: 10.1016/j.cmet.2022.11.013

Figure 1: Autochthonous metastatic mouse model of colon cancer is resistant to immunotherapy.

Figure 1:

A) Schematic of SingleMut, DoubleMut, and TripleMut. B) Mice were induced with one injection of 50 mg/kg tamoxifen and then assessed for survival (n=8–10), C) tumor number (n=8–10), D) tumor progression (n=8–10), and E) histology (n=8–10). F) Mice were treated with low dose tamoxifen and lungs and livers were macroscopically assessed for metastasis (n=7). G) Mice were induced with a tamoxifen dose calculated to cause death around 30 days in untreated mice for each model, treated daily with intraperitoneal anti-PD-L1 (1 μg per mouse) and monitored for survival. (n=8–10). Statistics were calculated with one-way ANOVA or t-test. Survival significance is calculated using log-rank test. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 unless otherwise indicated. Data is presented as mean ± SEM