Antibodies
|
Ki67 Antibody |
Cell Signaling |
Cat#12202T |
InVivoMAb anti-mouse CD8 |
BioXCell |
Cat#BE0117 |
Actin Antibody |
Protein Tech |
Cat# 60008-1-Ig |
OTC Polyclonal Antibody |
Invitrogen |
Cat#PA5-28197 |
CBS (D8F2P) Rabbit mAb |
Cell Signaling |
Cat#14782 |
Gamma Cystathionase Rabbit Polyclonal Antibody |
ProteinTech |
Cat#12217 |
Mat 1A/IIA Antibody (B-10) |
Santa Cruz |
Cat#166452 |
Rat Anti-Mouse IFN-Gamma |
BD Biosciences |
Cat# 554411 |
7-AAD |
BD Biosciences |
Cat# 559925 |
Rat Anti-Mouse TNF |
BD Biosciences |
Cat#561063 |
Cytof Antibodies |
Listed in Table 4. |
|
InVivoMAb anti-mouse PD-L1 |
BioXCell |
Cat#BE0101 |
Di-methyl-histone H3 (Lys9) (D85B4) Rabbit mAb |
Cell Signaling |
Cat#4658 |
Tri-methyl-histone H3 (Lys9) (D4W1U) Rabbit mAb |
Cell Signaling |
Cat#13969 |
Chemicals, Peptides, and Recombinant Proteins
|
Tamoxifen |
Sigma-Aldrich |
Cat#T5648 |
N-acetyl-cysteine |
Cayman Chemical |
Cat#20261 |
Sodium Sulfide |
Cayman Chemical |
Cat#10122555 |
Cystathionine |
Cayman Chemical |
Cat# 16061 |
Potassium Iodide |
Sigma |
Cat#7681-11-0 |
Mercury Iodide |
Sigma |
Cat# 221090 |
Ammonia Chloride |
Sigma |
Cat#213330 |
Potassium Hydroxide |
Sigma |
Cat#221473 |
Sodium Pyruvate |
Sigma |
Cat#P2256 |
Lactulose |
Sigma |
Cat#61360 |
Taurine |
Sigma |
Cat#T0625 |
Serine |
Sigma |
Cat#S4500 |
L-Ornithine Monohydrochloride |
Sigma |
Cat#O6503 |
Ferrostatin-1 |
Cayman Chemical |
Cat#17729 |
L-CC-Propargylglycine |
Sigma |
Cat#81838 |
Glutathione Ethyl Ester |
Cayman Chemical |
Cat#14953 |
Critical Commercial Assays
|
|
|
CellTrace CFSE Cell Proliferation Kit |
ThermoFisher |
Cat#C34554
|
Ammonia Assay Kit |
Sigma |
Cat#MAK310 |
Ammonia Assay Kit |
Sigma |
Cat#AA0100 |
DCFDA/H2DCFDA Cellular ROS Assay Kit |
AbCam |
Cat#ab113851 |
BODIPY (Lipid Peroxidation Sensor) |
ThermoFisher |
Cat#D3861 |
Experimental Models: Cell Lines
|
HCT116 |
ATCC |
N/A |
MC38 |
ATCC |
N/A |
CT26 |
ATCC |
N/A |
SW480 |
ATCC |
N/A |
Experimental Models: Organisms/Strains
|
Wildtype: C56Bl/6J |
Jackson Labs |
N/A |
SingleMut: CDX2-ERT2Cre; Apcfl/fl
|
Jackson Labs |
N/A |
DoubleMut: CDX2-ERT2Cre; Apcfl/fl; Trp53fl/
|
Jackson Labs |
N/A |
TripleMut: Apcfl/fl; Trp53fl/fl; KrasLSLG12D
|
Jackson Labs |
N/A |
Oligonucleotides
|
List of primers |
Supplemental Table 4
|
N/A |