Table 5. Comparison of Mean Values Before and After the Treatment Within the Same Group Regarding the Calcium/Phosphorus Ratio (paired t-test) .
| Group | Time | Mean | SD | Difference | t | P | |
| Mean | SD | ||||||
| Control | Before | 2.22 | 0.20 | -0.01 | 0.01 | -3.06 | 0.38 |
| After | 2.23 | 0.21 | |||||
| Eggshell NPs only | Before | 2.14 | 0.30 | -1.00 | 0.64 | -3.51 | 0.025* |
| After | 3.14 | 0.47 | |||||
| Seashell NPs only | Before | 2.09 | 0.13 | -0.91 | 0.08 | -24.65 | 0.000* |
| After | 3.00 | 0.19 | |||||
| Eggshell NPS + Nd:YAG laser | Before | 2.01 | 0.11 | -1.29 | 0.75 | -3.86 | 0.018* |
| After | 3.30 | 0.71 | |||||
| Seashell NPs + Nd:YAG laser | Before | 2.21 | 0.15 | -0.97 | 0.20 | -10.88 | 0.000* |
| After | 3.19 | 0.31 | |||||
Significance level P ≤ 0.05, *Significant.