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. 2022 Oct 22;51(1):e4. doi: 10.1093/nar/gkac940

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Characterization of hok suppressor substitutions. (A) Northern blot analysis of hok and Sok RNAs. The hok mRNA was revealed using a probe matching the hok mRNA 5′ UTR (FB178). The two FL forms of WT hok mRNA were detected in all transformants expressing hok. The translatable Tr-hok mRNA was present in most mutants, except for C345A and C358A. Sok was targeted with the probe FB213 and was absent in H*ΔS strains. 5S rRNA was revealed using probe FD211 and used as a loading control. P: promoter. (B) Western blotting of arabinose-induced Hok-FLAG protein mutants from pBAD plasmids (hok*-FLAG mRNA expression is stable; see Supplementary Figure S2). The upper signal corresponds to an unspecific band, as confirmed with pBAD vector control as well as induced (WT+) and uninduced (WT−) WT Hok-FLAG.