Table 1.
Summary of the localizations of single-nucleotide loss-of-function substitutions identified
| Location of the substitutions | Number of substitutions (number of positions) | Predicted mechanism of action |
|---|---|---|
| Promoter | 2 (1) | Transcription inhibition |
| hok mRNA 5′ UTR | 31 (22) | |
| 10 (4) | Destabilization of the tac-stem | |
| 7 (7) | Stabilization of aSD pairing | |
| 2 (2) | Mutation of mok SD sequence | |
| 12 (9) | Unknown | |
| mok CDS | 20 (18) | |
| 4 (2) | Mok and Hok not translated | |
| 5 (5) | Mok truncated and Hok not translated | |
| 11 (11) | Unknown | |
| hok CDS | 87 (64) | |
| 81 (59) | Hok activity impaired | |
| 3 (2) | Hok not translated | |
| 3 (3) | Unknown | |
| hok mRNA 3′ UTR | 22 (12) | |
| 19 (9) | Degradation by RNase | |
| 3 (3) | Unknown |
The locations of all significant substitutions obtained by FASTBAC-Seq are indicated as well as their number, and the number of nucleotide positions mutated is given in parentheses. The predicted mechanism of action for those mutations is indicated. See Supplementary Table S6 for details. CDS: coding sequence.