Figure 1.

LAMP Overview. Three primers (shown as small colored boxes) for each side of the target DNA to be amplified (black) are added with Bst polymerase and incubated at 65°C to start the reaction. During initiation, the primers anneal to their respective complementary regions in the target DNA, leading to extension of the forward and backward internal primers (FIP and BIP, shown with grey and yellow middle regions, respectively), which are then displaced by outer primers F3 and B3. Amplification of the displaced products in the opposite direction by the other internal primer results in structures that fold back to form loops, providing multiple opportunities for extension, ultimately leading to large concatemer formation and exponential amplification of the starting DNA.