Table 1.
Oligonucleotides used in this study
| Template | Oligo name | Sequence 5′—3′ (length in bp) | ∑260nm (M−1 cm−1) |
|---|---|---|---|
| GAPDH | FAM-FIP | [6-FAM]-CGCCAGTAGAGGCAGGGATGAGGGAAACTGTGGCGTGAT (39) | 413,560 |
| FAM-F3 | [6-FAM]-GCCACCCAGAAGACTGTG (18) | 194,860 | |
| FAM-LR | [6-FAM]-TGTTCTGGAGAGCCCCGCGGCC (22) | 217,260 | |
| LT-1 | TTGGCAGCGCCAGTAGAGGCAGGGATGATGTTCTGGAGAGCCCCGCGGCCATCACGCCACAGTTTCCCGGAGGGGCCATCCACAGTCTTCTGGGTGGCAG (100) | 940,500 | |
| LT-1C | CTGCCACCCAGAAGACTGTGGATGGCCCCTCCGGGAAACTGTGGCGTGATGGCCGCGGGGCTCTCCAGAACATCATCCCTGCCTCTACTGGCGCTGCCAA (100) | 912,700 | |
| LT-2 | CTCTCCAGAACATCATCCCTGCCTCTACTGGCGCTGCCAA (40) | 355,100 | |
| HT-1 | CCTTGGCAGCGCCAGTAGAGGCAGGGATGAGGGAAACTGTGGCGTGATGGCCGCGGGGCTCTCCAGAACATCATCCCTGCCTCTACTGGCGCTGCCAAGG (100) | 936,500 | |
| A. aegypti | FAM-BIP | [6-FAM]-TAGGTATAGACGTAGATACTCGAGCATTCCTGTAGGAACAGCAATA (46) | 492,660 |
| FAM-B3 | [6-FAM]-TTGAGTTCCGTGTAAAGTTG (20) | 215,760 | |
| FAM-LF | [6-FAM]-GCTTATTTTACTTCAGCAACTATAAT (26) | 269,860 | |
| HT-1 | TAGGTATAGACGTAGATACTCGAGCATTCCTGTAGGAACAGCAATAATTATAGTTGCTGAAGTAAAATAAGCTCGAGTATCTACGTCTATACCTA (95) | 963,300 | |
| LT-1 | TAGGTATAGACGTAGATACTCGAGCTTATTTTACTTCAGCAACTATAATTATTGCTGTTCCTACAGGAATTAAAATTTTTAGTTGATTAGCAACTTTACACGGAACTCAA (110) | 1,086,500 | |
| LT-1C | TTGAGTTCCGTGTAAAGTTGCTAATCAACTAAAAATTTTAATTCCTGTAGGAACAGCAATAATTATAGTTGCTGAAGTAAAATAAGCTCGAGTATCTACGTCTATACCTA (110) | 1,102,500 | |
| - | Cy3 Internal Standard | [Cy3]-CCGTGAGTTGGTTGGACGGCTGCGAGGC (28) | 266,800 |