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. 2022 Dec 30;51(1):337–348. doi: 10.1093/nar/gkac1200

Figure 4.

Figure 4.

Unwinding activity of WRN on DNA/RNA hybrid substrates. (A) DNA/RNA hybrid sample with a 3′-overhang RNA strand (left). The RNA region is indicated by cyan. Representative fluorescence intensity time traces of Cy3 (green) and Cy5 (red) in the presence of 1 mM ATP and 1nM RPA (right). No molecule (0/698) shows unwinding behavior. 26 nM wild type WRN was used. (B) DNA/RNA hybrid sample with a 5′-overhang RNA strand (left). Representative intensity time traces of Cy3 (green) and Cy5 (red) in the presence of 1 mM ATP and 1nM RPA (right). 30.9% (224/725) of molecules show unwinding behavior. 26 nM wild type WRN was used.