Figure 6.

RNA cytometry of acute promyelocytic leukemia cells and human peripheral blood mononuclear cells. Upper and bottom rows exhibit image-based and FACS-based RNA cytometry of human PBMCs spiked with different dilutions of NB-4 cells, respectively. The multiplex scRT-PCR targeted PTPRC (CD45) and PML-RARα transcripts. The PBMC cells were detected by expression of marker gene PTPRC, the NB-4 cells were quantified by recording the co-expression of PTPRC and PML-RARα. The percentages depict the fraction of total counts. Refer to accompanying Supplementary Figure S13 for the gating strategy.