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. 2022 Dec 22;51(1):271–289. doi: 10.1093/nar/gkac1175

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Detection of distinct peptide products by translation of two ORFs from an AUGUG ambiguous start site. (A) Schematic representation of the reporter plasmids for detection of translation products from either potential ORF. The RBS of the reporter sequences is depicted with the 8 nt strong SD motif (blue) and the spacer sequence (grey). (B) Autoradiogram of radiolabeled in vitro translation (IVT) products from the reporter constructs depicted in (A), including a reaction without template as negative control (control).