Figure 2.

Intramacrophage killing activity of selected nNF derivatives against M. tuberculosis H37Rv. BMDM were infected with M. tuberculosis H37Rv at a MOI of 1, and viable intracellular bacteria were enumerated by CFU after 6 days. The Y axis represents the total number of CFU calculated per well for the different experimental groups: untreated infected control (black), and infected and treated with nNF-C2 (blue), nNF-C4 (orange), nNF-C20 (yellow) and RIF (grey). Two to 32 times of the corresponding in vitro MIC₉₀ obtained for each compound were the concentrations used for the intracellular bactericidal assessment. Exact assayed concentrations for each compound are displayed in μg/ml. CFU values are represented as the mean ± SD of 3 independent experiments.