Table 2.
Cytotoxicity assessment of nNFs
| M. tuberculosis | VERO cells | |||
|---|---|---|---|---|
| Compound | MIC90 (μg/ml) | CC50 (μg/ml) | SI | |
| High activity | nNF-C1 | 0.125 | 69.2 | 553.6 |
| nNF-C2 | 0.0625 | 75.7 | 1211.5 | |
| nNF-C3 | 1 | 21.5 | 21.5 | |
| nNF-C4 | 0.125 | 64.9 | 519.2 | |
| nNF-C5 | 0.25 | 12.5 | 50.0 | |
| nNF-C6 | 0.5 | 91.3 | 182.6 | |
| nNF-C8 | 0.125 | 0.4 | 3.4 | |
| nNF-C16 | 0.0625 | 0.3 | 5.2 | |
| nNF-C18 | 0.25 | 2.2 | 8.8 | |
| nNF-C20 | 0.25 | 9.3 | 37.1 | |
| Moderate activity | nNF-C7 | 4 | 67.5 | 16.9 |
| nNF-C11 | 8 | >100 | >12,5 | |
| nNF-C12 | 4 | 73.5 | 18.4 | |
| nNF-C14 | 2 | >100 | >50 | |
| nNF-C17 | 2 | 55.1 | 27.5 | |
| RIF | 0.01 | 78.2 | 7824.1 |
Cytotoxicity of the nNF derivatives classified as highly or moderately active against M. tuberculosis was determined in Vero cells after 72 h of chemical exposition. Cellular viability was determined by indirectly measuring the amount of ATP. CC50 was calculated as the concentration at which 50% of the cells were viable compared to the untreated control. MIC90 and CC50 values are expressed in μg/ml. The selectivity index (SI) is expressed as CC50/MIC90.