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. 2022 Dec 22;51(1):449–462. doi: 10.1093/nar/gkac1189

Figure 6.

Figure 6.

Ribosome-bound THR likely prevents binding of release factor to the A site. (A) Superimposition of the ribosome-bound RF1 (PDB entry 3D5A (57)) with the new structure of 70S-THR complex carrying both A- and P-site tRNAs. The structures were aligned based on helix 44 of the 16S rRNA. Note that the THR-induced flipped-out conformation of C1914 would cause steric clashes with RF1 bound in the A site. (B) Structural re-arrangements in the decoding center that are required for RF1 to bind to the 70S ribosome (dashed red arrows). Note that binding of RF1/2 to the ribosome requires nucleotides A1492, A1493 of the 16S rRNA and A1913 of the 23S rRNA to adopt positions that are incompatible with ribosome-bound THR.