Table 2.
Assembly statistics
| Assembly | Size (Mb) | Contigs | N50 | Avg_len | BUSCO | Cov | Technique | Assembler |
|---|---|---|---|---|---|---|---|---|
| C. floridanus_7.5 | 284 009 182 | 657 | 1 278 439 | 432 282 | 96.7 | 53× | PacBio | Canu |
| C. floridanus_1.0 | 232 685 334 | 10 791 | 19 487 | 21 563 | 96.0 | 100× | Illumina | SOAPdenovo v. 1.0 |
| C. pennsylvanicus assembled using all reads | ||||||||
| NextDenovo | 278 594 827 | 1225 | 560 840 | 227 424 | 90.1 | Nanopore | NextDenovo 2.5.0 | |
| Shasta | 281 462 227 | 3277 | 430 265 | 85 890 | 94.1 | Nanopore | Shasta 0.8.0 | |
| Raven | 295 081 168 | 1774 | 333 350 | 166 337 | 95.7 | Nanopore | Raven 1.8.1 | |
| Flye | 272 267 557 | 5516 | 520 016 | 49 360 | 96.8 | Nanopore | Flye 2.9-b1768 | |
| C. pennsylvanicus assembled using reads >1000 bp | ||||||||
| Flye | 309 753 621 | 1655 | 565 278 | 187 162 | 96.9 | Nanopore | Flye | |
| Flye-Racon-1X | 309 865 371 | 1648 | 565 400 | 187 681 | 96.9 | Nanopore | Flye + racon_1X | |
| Flye-Racon-2X | 309 298 248 | 1641 | 565 935 | 188 189 | 96.8 | Nanopore | Flye + racon_2X | |
| Flye-Racon-3X | 308 443 459 | 1633 | 565 664 | 188 882 | 96.9 | Nanopore | Flye + racon_3X | |
| Flye-Racon-4X | 308 109 057 | 1625 | 565 657 | 189 606 | 97.0 | Nanopore | Flye + racon_4X | |
| Flye-R4X-Medaka | 308 881 295 | 1625 | 566 325 | 190 081 | 97.0 | Nanopore | Flye + R4X + Medaka1X | |
| Final assembly | 306 426 343 | 1609 | 565 603 | 190 445 | 97.0 | 60× | Nanopore | Manually curated |