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. Author manuscript; available in PMC: 2023 Jan 16.
Published in final edited form as: ACS Biomater Sci Eng. 2020 Nov 4;6(12):6808–6818. doi: 10.1021/acsbiomaterials.0c00788

Figure 1.

Figure 1.

Differentiation of cardiomyocytes from hiPSCs and cell sheet generation. (A) The pluripotency markers (Nanog, OCT4, SOX2, TRA-60, SSEA4) and alkaline phosphatase activity expressions in the cultured hiPSCs. (B) Experiment timeline of the cardiomyocyte differentiation procedure and representative phase contrast images showing the morphological changes of the cells during the process. Areas defined by yellow dotted line consist of spontaneous beating cells. (C) Cardiac-specific markers (GATA4, cTnT, MLC-2v, calponin) expressions in the hiPSCs-derived CMs. (D) The fluorescence intensity changes of fluo-4 (calcium indicator) during relaxing and contracting stages of the hiPSC-derived CMs both in the center and at the periphery of the beating area. F0 is the fluo-4 fluorescence during relaxing stage. n = 3 (E) Detachment of cardiomyocyte sheet from a thermal responsive cell culture plate by lowering the culture temperature from 37 °C to room temperature for 2 h. Scale bar: 100 μm.