Figure 3.

Illustration of rebound current magnitudes and residual ACh activity following the application of compounds of interest. (A) Magnitudes of rebound currents appearing after the cessation of test compound application were recorded from α7- and α9α10-nAChRs. As for Figure 2, responses at both α7- and α9α10-nAChR were quantified in terms of both peak currents and AUC. (B) Residual activation evoked by a final ACh control application (1 mM, 1 s) applied 1 min after stimulation with each compound of interest. In this case, too, responses at both nAChR subtypes were measured as both peak current and AUC and, in each case, normalized to the ACh control responses that preceded the application of the test compound. Each bar represents the mean response collected from three to seven individual oocytes, with error bars representing the S.E.M. Points represent responses from individual oocytes.