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Stimulation of cAMP accumulation by unlabeled and Cy5-labeled PACAP-38, PACAP-27, and VIP in Cos7 cells transfected with the PAC1n, PAC1s, VPAC1, or VPAC2 receptor. Data were normalized to the maximal responses produced by the corresponding unlabeled peptide and expressed as a percentage. Data points are the mean ± SEM of the combined data from 5 independent experiments.
