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. 2022 Dec 7;6(1):76–87. doi: 10.1021/acsptsci.2c00165

Figure 5.

Figure 5

Peptide KKP1 treatment induces the transcriptional activity of the Nrf2/ARE signaling pathway. (A) Schematic representation of the Nrf2 luciferase reporter constructs shown in the top panel, while the bottom panel represents the experimental protocol of this assay. (B) Relative luciferase activity in HSC-T6 cells transfected with the Nrf2/ARE reporter constructs undergoing KKP1, KKP2, and KKPs treatment for 4, 8, 16, and 24 h. (C) Corresponding p-value plot of the data pairs shown in (B). No significance of the 4 h treatment was shown. (D) Bar graph shows the AUC analysis of (B). (E) Western blot analysis of the GCLC and HO-1 protein levels in HSC-T6 cells incubated with KKP1, KKP2, and KKPs for 8 h. (F) Densitometric quantification of the GCLC and HO-1 proteins in the blots shown in (E). Data in the graph are means ± SEM of three independent measurements. (G) Corresponding p-value plot of the data pairs shown in (F).