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. 2022 Dec 30;6(1):151–170. doi: 10.1021/acsptsci.2c00202

Figure 6.

Figure 6

Binding of ML321 to the D2R is regulated by Na+. D2R and D3R radioligand binding assays were performed as described in the Methods using Tris buffer in the absence or presence of 140 mM NaCl. The data are expressed as percentage of the control specific binding and represent mean ± SEM values from three independent experiments each performed in triplicate. Mean ML321 Ki values [95% C.I.] for each receptor were calculated from the IC50 values using the Cheng–Prusoff equation.31 (A) D2R competition binding curves ± NaCl. In the presence of Na+, the mean ML321 Ki = 72.5 nM [56.3–93.3] whereas in the absence of Na+ the Ki >10 μM. (B) D3R competition binding curves ± NaCl. In the absence or presence of Na+, the ML321 Ki values are >10 μM.