Fig. 3.

Representative image of immunohistochemical staining of liver sections with anti-caspase-3 antibody from various studied groups (A) Control (B) HFD+T2DM group (C) HFD+T2DM+Fasudil group. Arrowhead points to the brown coloration of the immuno-positive cells. (D) Histogram shows the % area of immuno-positive cells from the various experimental groups. HFD+T2DM group showed significant increase in caspase-3 immunostaining compared to other groups. HFD+T2DM+Fasudil group revealed weakly positive immunostaining.