Table 2.
The levels of glutathione (GSH), total antioxidant activity (TAC), nitric oxide (NO) and thiobarbituric acid-reactive substances (TBARS) and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione S-transferase (GST), in the brain, heart and lung tissues of male rats treated with aluminum oxide nanoparticles (Al2O3-nps), zinc oxide nanoparticles (ZnO-nps) and their combination.
| Parameter | Experimental group | |||
|---|---|---|---|---|
|
| ||||
| Control | Al2O3-nps | ZnO-nps | Al2O3-nps + ZnO-nps | |
| Brain | ||||
| GSH (μmol/g tissue) | 5.6±0.09a | 3.3±0.10b | 3.0±0.13b | 2.4±0.13c |
| SOD (mU/mg protein) | 49.3±2.6a | 28.4±2.8b | 13.2±0.8c | 8.0±0.8d |
| CAT (mU/mg protein) | 40±1.66a | 24±1.27b | 16±0.80c | 12±0.90d |
| GPx (mU/mg protein) | 56.5±3.20a | 41.3±2.1b | 32.3±1.15c | 20.9±2.38d |
| GST (U/mg protein) | 0.71±0.02a | 0.56±0.02b | 0.36±0.01c | 0.25±0.01d |
| TAC (μM/g tissue) | 20.03±0.36a | 17.40±0.25b | 14.51±0.39b | 12.37±0.54c |
| NO (μmol/g tissue) | 0.43±0.02d | 0.57±0.01c | 0.84±0.01b | 1.16±0.03a |
| TBARS (nmol/g tissue) | 35±0.9d | 62±0.6c | 73±3.3b | 90±0.8a |
|
| ||||
| Heart | ||||
| GSH (μmol/g tissue) | 5.1±0.08a | 3.0±0.04b | 2.6±0.06c | 2.0±0.03d |
| SOD (mU/mg protein) | 28.7±2.6a | 25.7±2.8b | 13.3±1.2c | 8.6±0.8d |
| CAT (mU/mg protein) | 59±4.20a | 31±5.61b | 27±4.37b | 14±1.80c |
| GPx (mU/mg protein) | 56.3±1.738a | 34.6±0.647b | 24.8±1.255c | 17.2±0.417d |
| GST (U/mg protein) | 0.50±0.01a | 0.40±0.01b | 0.34±0.0c | 0.28±0.0c |
| TAC (μM/g tissue) | 2.31±0.15a | 1.73±0.05b | 1.71±0.03b | 1.38±0.01c |
| NO (μmol/g tissue) | 0.28±0.01c | 0.33±0.00c | 0.44±0.01b | 0.50±0.01a |
| TBARS (nmol/g tissue) | 61.9±0.9d | 104±0.7b | 90±2.1c | 113±0.5a |
|
| ||||
| Lung | ||||
| GSH (μmol/g tissue) | 7.4±0.14a | 6.5±0.12b | 5.7±0.20c | 4.8±0.08d |
| SOD (mU/mg protein) | 10.4±0.6a | 6.0±0.8b | 4.7±0.8c | 3.8±0.2d |
| CAT (mU/mg protein) | 62±2.59a | 46±2.24b | 31±1.58c | 23±0.82d |
| GPx (m/mg protein) | 58.8±1.95a | 48.1±2.86b | 41.8±2.15c | 17.1±0.89d |
| GST (U/mg protein) | 0.75±0.0a | 0.51±0.01b | 0.49±0.0c | 0.40±0.0d |
| TAC (μM/g tissue) | 2.37±0.01a | 2.11±0.02b | 1.87±0.01c | 1.63±0.01d |
| NO (μmol/g tissue) | 0.4±0.01d | 0.5±0.01c | 0.7±0.01b | 0.8±0.01a |
| TBARS (nmol/g tissue) | 17±0.2d | 20±0.3c | 22±0.6b | 32±0.6a |
Mean values within a row not sharing common superscripts (a, b, c, d) were significantly different, p<0.05.